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PCR Laboratory Project of Wuhan Puren Hospital

Wuhan Puren Hospital is located at No.1 Benxi Street, Qingshan District, Wuhan City, Hubei Province, founded in 1955, is a Grade 3A comprehensive hospital integrating medical treatment, prevention, scientific research, teaching and rehabilitation, and is a designated hospital for Wuhan health insurance. On September 21, 2020, it was named as an advanced collective in Hubei Province for fighting against the new crown pneumonia epidemic. ​On November 28, 2023, the Wuhan Municipal Health Commission of Hubei Province issued a circular: it has suspended the qualification of Wuhan Puren Hospital in issuing birth medical certificates.

Project address
Wuhan, China
Industry
Lab
Building area
200m²
ISO Class
ISO7
Temperature
22-26℃
Humidity
45%-65%

Client Profile

Wuhan Puren Hospital, founded in 1955 and located at No. 1 Benxi Street, Qingshan District, Wuhan City, Hubei Province, is a comprehensive tertiary-level hospital integrating medical treatment, prevention, scientific research, teaching, and rehabilitation. It is an affiliated hospital of Wuhan University of Science and Technology. The hospital has a complete range of departments and handles over one million outpatient visits annually. It is a national "Chest Pain Center," "Heart Failure Center," "Stroke Center," and a "Baby-Friendly Hospital" [1]. In 2020, the hospital was recognized as an advanced collective in Hubei Province for its efforts in combating the COVID-19 pandemic [2]. In 2023, the hospital was investigated for issues related to surrogacy, and its authority to issue birth certificates was suspended [7]. In 2024, it was fined for medical management issues.

Layout diagram

3D diagram of the Puren hospital  PCR laboratory

Project Overview

Hospital:
Puren Hospital
Laboratory Type:
PCR (Polymerase Chain Reaction) Laboratory
Design & Construction:
Deiiang Company
Project Period:
2019 - 2020

This PCR laboratory construction project was initiated in 2019 to meet the growing demand for molecular diagnostic testing.                The facility is designed to biosafety level II (BSL-2) standards with separate zones for specimen processing, nucleic acid extraction,                amplification, and analysis to prevent contamination.

Construction Timeline

Mar-Apr 2019

1
Project Design

Initial planning and design phase completed by Deiiang Company. Includes architectural layout,                                workflow design for PCR process (pre-PCR, PCR, post-PCR areas), biosafety planning, and equipment placement.

Design complies with BSL-2 requirements and PCR laboratory contamination prevention guidelines.
May-Jun 2019

2
Civil Structure Renovation

Structural modifications to existing hospital space. Includes wall demolition, new partition construction,                                ceiling adjustments, and preparation of service penetrations for utilities.

Structural reinforcement completed to support specialized laboratory equipment.
Jul 2019

3
Clean Room Decoration

Installation of clean room panels, ceilings with sealed lighting fixtures, airtight doors with viewing windows,                                and pass-through chambers. All surfaces designed for easy cleaning and decontamination.

Materials selected for chemical resistance and minimal particle shedding.
Aug 2019

4
HVAC System Installation

Installation of dedicated heating, ventilation, and air conditioning system with HEPA filtration.                                Maintains positive/negative pressure gradients between different laboratory zones to prevent cross-contamination.

System maintains temperature at 22±2°C and relative humidity at 50±10%.
Sep 2019

5
Exhaust System Installation

Installation of dedicated exhaust system for biological safety cabinets, fume hoods, and general laboratory exhaust.                                Includes HEPA filtration at exhaust points and proper discharge away from air intakes.

System ensures 12-15 air changes per hour in laboratory areas.
Oct 2019

6
Flooring Construction

Installation of seamless epoxy flooring with chemical resistance and anti-static properties.                                Flooring includes integrated coving at wall junctions to eliminate corners and facilitate cleaning.

Flooring meets ISO Class 7 cleanroom standards with low particulate emission.
Nov 2019

7
Plumbing & Drainage

Installation of laboratory-grade plumbing including pure water system, emergency eyewash stations,                                handwashing sinks, and chemical-resistant drainage with proper traps and vents.

System includes backflow prevention and appropriate waste handling for laboratory effluents.
Dec 2019

8
Electrical System Installation

Installation of dedicated electrical circuits, UPS systems, emergency power, laboratory-grade outlets,                                and specialized lighting. Includes data cabling for laboratory equipment and computer networks.

Electrical design includes isolated grounding for sensitive instruments and surge protection.
Jan 2020

9
Equipment Installation

Installation and calibration of PCR laboratory equipment including biological safety cabinets,                                centrifuges, thermal cyclers, real-time PCR instruments, nucleic acid extractors, and freezers.

Equipment arranged following unidirectional workflow to prevent PCR product contamination.
Feb-Mar 2020

10
Testing & Certification

Comprehensive testing of all systems including air cleanliness validation, pressure differential verification,                                HEPA filter integrity testing, equipment calibration, and workflow validation.

Facility certified to meet BSL-2 standards and ready for clinical PCR testing operations.

Jiangxi Construction Engineering

This project was handled by our experienced cleanroom construction team, which consisted of 5 project managers, 15 engineers, and 40 skilled workers. All members hold relevant certifications and have received professional cleanroom construction training, ensuring project quality and timely completion.

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